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g body weight αccl11  (R&D Systems)


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    R&D Systems g body weight αccl11
    Increased VAT-T R accumulation in the absence of ICOS signaling is associated with elevated CCR3 expression. (A) ST2 expression in splenic T R s ( n = 3–5 per group from eight independent experiments). (B) ST2 expression in CD45.1 + and CD45.2 + donor splenic T R s in WT:YF and WT:KO chimeric mice. Lines connect CD45.1 + and CD45.2 + donor splenic T R s within the same chimera ( n = 3–5 chimeric mice per group from three independent experiments). (C) Expression of CCR3 in VAT-T R s in mice ≤8 wk and >8 wk of age ( n = 3–5 per group from seven independent experiments). (D) Expression of CCR2 and CCR3 by VAT-T R s ( n = 3–5 per group from two independent experiments). DP, CCR2 + CCR3 + ; DN, CCR2 − CCR3 − . (E) Expression of indicated CCR3 ligands in total VAT normalized to Tbp as measured by qPCR (n.d. indicates not detected; n = 5 per group). (F) Schematic of in vitro culture experiments examining the impact of ICOS signaling on CCR3 expression (left). Graphs indicating fold change in T R frequency of CD4 + cells and %CCR3 + of T R s between individual culture samples stimulated (stim) with or without αICOS for 2 d (middle). Representative flow cytometry plots with frequency of CCR3 + T R s after 2 d in specified culture conditions (right; n = 1–3 per group from three independent experiments). (G) Left: Representative flow cytometry plots indicating VAT-T R frequency with or without CCL11/24 blockade. Graphs summarize T R frequencies in indicated tissues after 2 wk ( n = 3–4 per group). Mice were age matched within independent experiments and collectively; pooled data are from experiments using male mice aged 8–16 wk unless otherwise indicated. Statistical significance was determined using one-way ANOVA with Tukey’s post-test (A and C); two-tailed, paired Student’s t test for expression in donor cells within the same chimeric mouse (B); and two-tailed Student’s t test (F and G). All data are presented as mean values ± SD.
    G Body Weight αccl11, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/g+body+weight+%CE%B1ccl11/pmc08065270-260-7-16?v=R%26D+Systems
    Average 93 stars, based on 12 article reviews
    g body weight αccl11 - by Bioz Stars, 2026-07
    93/100 stars

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    1) Product Images from "ICOS signaling limits regulatory T cell accumulation and function in visceral adipose tissue"

    Article Title: ICOS signaling limits regulatory T cell accumulation and function in visceral adipose tissue

    Journal: The Journal of Experimental Medicine

    doi: 10.1084/jem.20201142

    Increased VAT-T R accumulation in the absence of ICOS signaling is associated with elevated CCR3 expression. (A) ST2 expression in splenic T R s ( n = 3–5 per group from eight independent experiments). (B) ST2 expression in CD45.1 + and CD45.2 + donor splenic T R s in WT:YF and WT:KO chimeric mice. Lines connect CD45.1 + and CD45.2 + donor splenic T R s within the same chimera ( n = 3–5 chimeric mice per group from three independent experiments). (C) Expression of CCR3 in VAT-T R s in mice ≤8 wk and >8 wk of age ( n = 3–5 per group from seven independent experiments). (D) Expression of CCR2 and CCR3 by VAT-T R s ( n = 3–5 per group from two independent experiments). DP, CCR2 + CCR3 + ; DN, CCR2 − CCR3 − . (E) Expression of indicated CCR3 ligands in total VAT normalized to Tbp as measured by qPCR (n.d. indicates not detected; n = 5 per group). (F) Schematic of in vitro culture experiments examining the impact of ICOS signaling on CCR3 expression (left). Graphs indicating fold change in T R frequency of CD4 + cells and %CCR3 + of T R s between individual culture samples stimulated (stim) with or without αICOS for 2 d (middle). Representative flow cytometry plots with frequency of CCR3 + T R s after 2 d in specified culture conditions (right; n = 1–3 per group from three independent experiments). (G) Left: Representative flow cytometry plots indicating VAT-T R frequency with or without CCL11/24 blockade. Graphs summarize T R frequencies in indicated tissues after 2 wk ( n = 3–4 per group). Mice were age matched within independent experiments and collectively; pooled data are from experiments using male mice aged 8–16 wk unless otherwise indicated. Statistical significance was determined using one-way ANOVA with Tukey’s post-test (A and C); two-tailed, paired Student’s t test for expression in donor cells within the same chimeric mouse (B); and two-tailed Student’s t test (F and G). All data are presented as mean values ± SD.
    Figure Legend Snippet: Increased VAT-T R accumulation in the absence of ICOS signaling is associated with elevated CCR3 expression. (A) ST2 expression in splenic T R s ( n = 3–5 per group from eight independent experiments). (B) ST2 expression in CD45.1 + and CD45.2 + donor splenic T R s in WT:YF and WT:KO chimeric mice. Lines connect CD45.1 + and CD45.2 + donor splenic T R s within the same chimera ( n = 3–5 chimeric mice per group from three independent experiments). (C) Expression of CCR3 in VAT-T R s in mice ≤8 wk and >8 wk of age ( n = 3–5 per group from seven independent experiments). (D) Expression of CCR2 and CCR3 by VAT-T R s ( n = 3–5 per group from two independent experiments). DP, CCR2 + CCR3 + ; DN, CCR2 − CCR3 − . (E) Expression of indicated CCR3 ligands in total VAT normalized to Tbp as measured by qPCR (n.d. indicates not detected; n = 5 per group). (F) Schematic of in vitro culture experiments examining the impact of ICOS signaling on CCR3 expression (left). Graphs indicating fold change in T R frequency of CD4 + cells and %CCR3 + of T R s between individual culture samples stimulated (stim) with or without αICOS for 2 d (middle). Representative flow cytometry plots with frequency of CCR3 + T R s after 2 d in specified culture conditions (right; n = 1–3 per group from three independent experiments). (G) Left: Representative flow cytometry plots indicating VAT-T R frequency with or without CCL11/24 blockade. Graphs summarize T R frequencies in indicated tissues after 2 wk ( n = 3–4 per group). Mice were age matched within independent experiments and collectively; pooled data are from experiments using male mice aged 8–16 wk unless otherwise indicated. Statistical significance was determined using one-way ANOVA with Tukey’s post-test (A and C); two-tailed, paired Student’s t test for expression in donor cells within the same chimeric mouse (B); and two-tailed Student’s t test (F and G). All data are presented as mean values ± SD.

    Techniques Used: Expressing, In Vitro, Flow Cytometry, Two Tailed Test

    Increased accumulation of CCR3 + T R s in the absence of ICOS signaling is specific to VAT (goes with ). (A) CCR2 expression by splenic (top) and VAT-T R s (bottom) as measured by flow cytometry ( n = 3–5 per group from two independent experiments). (B) Expression of CCR3 in splenic T R s in mice ≤8 wk and >8 wk of age ( n = 3–5 per group from seven independent experiments). (C) Expression of CCR3 by gated CD45.1 + and CD45.2 + donor VAT-T R s in chimeric mice. Graphs summarize CCR3 expression by donor T R s in VAT and spleen. Line connects point representing CD45.1 + and CD45.2 + cells within the same chimeric mouse ( n = 2–4 per group from two independent experiments). (D) CCR3 expression by tissue-localized skin T R s as measured by flow cytometry ( n = 2–4 per group from two independent experiments). (E) CCR3 expression by CD45.1 + and CD45.2 + donor T R s within the same chimeric mouse in indicated tissues. Line connects CD45.1 + and CD45.2 + cells within the same chimeric mouse ( n = 2–4 per group from two independent experiments). (F) Frequency of tissue-restricted VAT eosinophils with and without in vivo CCL11/24 blockade as measured by flow cytometry ( n = 3 or 4 mice per group). Mice were age matched within individual experiments, and pooled data are from experiments using 8–16-wk-old male mice. Statistical significance was determined using one-way ANOVA with Tukey’s post-test (A, B, and D); two-tailed, paired Student’s t test for expression in donor cells within the same chimeric mouse (C and E); and two-tailed Student’s t test (F). All data are presented as mean values ± SD. LPL, lamina propria lymphocyte.
    Figure Legend Snippet: Increased accumulation of CCR3 + T R s in the absence of ICOS signaling is specific to VAT (goes with ). (A) CCR2 expression by splenic (top) and VAT-T R s (bottom) as measured by flow cytometry ( n = 3–5 per group from two independent experiments). (B) Expression of CCR3 in splenic T R s in mice ≤8 wk and >8 wk of age ( n = 3–5 per group from seven independent experiments). (C) Expression of CCR3 by gated CD45.1 + and CD45.2 + donor VAT-T R s in chimeric mice. Graphs summarize CCR3 expression by donor T R s in VAT and spleen. Line connects point representing CD45.1 + and CD45.2 + cells within the same chimeric mouse ( n = 2–4 per group from two independent experiments). (D) CCR3 expression by tissue-localized skin T R s as measured by flow cytometry ( n = 2–4 per group from two independent experiments). (E) CCR3 expression by CD45.1 + and CD45.2 + donor T R s within the same chimeric mouse in indicated tissues. Line connects CD45.1 + and CD45.2 + cells within the same chimeric mouse ( n = 2–4 per group from two independent experiments). (F) Frequency of tissue-restricted VAT eosinophils with and without in vivo CCL11/24 blockade as measured by flow cytometry ( n = 3 or 4 mice per group). Mice were age matched within individual experiments, and pooled data are from experiments using 8–16-wk-old male mice. Statistical significance was determined using one-way ANOVA with Tukey’s post-test (A, B, and D); two-tailed, paired Student’s t test for expression in donor cells within the same chimeric mouse (C and E); and two-tailed Student’s t test (F). All data are presented as mean values ± SD. LPL, lamina propria lymphocyte.

    Techniques Used: Expressing, Flow Cytometry, In Vivo, Two Tailed Test



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    g body weight αccl11  (R&D Systems)
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    R&D Systems g body weight αccl11
    Increased VAT-T R accumulation in the absence of ICOS signaling is associated with elevated CCR3 expression. (A) ST2 expression in splenic T R s ( n = 3–5 per group from eight independent experiments). (B) ST2 expression in CD45.1 + and CD45.2 + donor splenic T R s in WT:YF and WT:KO chimeric mice. Lines connect CD45.1 + and CD45.2 + donor splenic T R s within the same chimera ( n = 3–5 chimeric mice per group from three independent experiments). (C) Expression of CCR3 in VAT-T R s in mice ≤8 wk and >8 wk of age ( n = 3–5 per group from seven independent experiments). (D) Expression of CCR2 and CCR3 by VAT-T R s ( n = 3–5 per group from two independent experiments). DP, CCR2 + CCR3 + ; DN, CCR2 − CCR3 − . (E) Expression of indicated CCR3 ligands in total VAT normalized to Tbp as measured by qPCR (n.d. indicates not detected; n = 5 per group). (F) Schematic of in vitro culture experiments examining the impact of ICOS signaling on CCR3 expression (left). Graphs indicating fold change in T R frequency of CD4 + cells and %CCR3 + of T R s between individual culture samples stimulated (stim) with or without αICOS for 2 d (middle). Representative flow cytometry plots with frequency of CCR3 + T R s after 2 d in specified culture conditions (right; n = 1–3 per group from three independent experiments). (G) Left: Representative flow cytometry plots indicating VAT-T R frequency with or without CCL11/24 blockade. Graphs summarize T R frequencies in indicated tissues after 2 wk ( n = 3–4 per group). Mice were age matched within independent experiments and collectively; pooled data are from experiments using male mice aged 8–16 wk unless otherwise indicated. Statistical significance was determined using one-way ANOVA with Tukey’s post-test (A and C); two-tailed, paired Student’s t test for expression in donor cells within the same chimeric mouse (B); and two-tailed Student’s t test (F and G). All data are presented as mean values ± SD.
    G Body Weight αccl11, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/g+body+weight+%CE%B1ccl11/pmc08065270-260-7-16?v=R%26D+Systems
    Average 93 stars, based on 1 article reviews
    g body weight αccl11 - by Bioz Stars, 2026-07
    93/100 stars
    		  Buy from Supplier

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    Increased VAT-T R accumulation in the absence of ICOS signaling is associated with elevated CCR3 expression. (A) ST2 expression in splenic T R s ( n = 3–5 per group from eight independent experiments). (B) ST2 expression in CD45.1 + and CD45.2 + donor splenic T R s in WT:YF and WT:KO chimeric mice. Lines connect CD45.1 + and CD45.2 + donor splenic T R s within the same chimera ( n = 3–5 chimeric mice per group from three independent experiments). (C) Expression of CCR3 in VAT-T R s in mice ≤8 wk and >8 wk of age ( n = 3–5 per group from seven independent experiments). (D) Expression of CCR2 and CCR3 by VAT-T R s ( n = 3–5 per group from two independent experiments). DP, CCR2 + CCR3 + ; DN, CCR2 − CCR3 − . (E) Expression of indicated CCR3 ligands in total VAT normalized to Tbp as measured by qPCR (n.d. indicates not detected; n = 5 per group). (F) Schematic of in vitro culture experiments examining the impact of ICOS signaling on CCR3 expression (left). Graphs indicating fold change in T R frequency of CD4 + cells and %CCR3 + of T R s between individual culture samples stimulated (stim) with or without αICOS for 2 d (middle). Representative flow cytometry plots with frequency of CCR3 + T R s after 2 d in specified culture conditions (right; n = 1–3 per group from three independent experiments). (G) Left: Representative flow cytometry plots indicating VAT-T R frequency with or without CCL11/24 blockade. Graphs summarize T R frequencies in indicated tissues after 2 wk ( n = 3–4 per group). Mice were age matched within independent experiments and collectively; pooled data are from experiments using male mice aged 8–16 wk unless otherwise indicated. Statistical significance was determined using one-way ANOVA with Tukey’s post-test (A and C); two-tailed, paired Student’s t test for expression in donor cells within the same chimeric mouse (B); and two-tailed Student’s t test (F and G). All data are presented as mean values ± SD.

    Journal: The Journal of Experimental Medicine

    Article Title: ICOS signaling limits regulatory T cell accumulation and function in visceral adipose tissue

    doi: 10.1084/jem.20201142

    Figure Lengend Snippet: Increased VAT-T R accumulation in the absence of ICOS signaling is associated with elevated CCR3 expression. (A) ST2 expression in splenic T R s ( n = 3–5 per group from eight independent experiments). (B) ST2 expression in CD45.1 + and CD45.2 + donor splenic T R s in WT:YF and WT:KO chimeric mice. Lines connect CD45.1 + and CD45.2 + donor splenic T R s within the same chimera ( n = 3–5 chimeric mice per group from three independent experiments). (C) Expression of CCR3 in VAT-T R s in mice ≤8 wk and >8 wk of age ( n = 3–5 per group from seven independent experiments). (D) Expression of CCR2 and CCR3 by VAT-T R s ( n = 3–5 per group from two independent experiments). DP, CCR2 + CCR3 + ; DN, CCR2 − CCR3 − . (E) Expression of indicated CCR3 ligands in total VAT normalized to Tbp as measured by qPCR (n.d. indicates not detected; n = 5 per group). (F) Schematic of in vitro culture experiments examining the impact of ICOS signaling on CCR3 expression (left). Graphs indicating fold change in T R frequency of CD4 + cells and %CCR3 + of T R s between individual culture samples stimulated (stim) with or without αICOS for 2 d (middle). Representative flow cytometry plots with frequency of CCR3 + T R s after 2 d in specified culture conditions (right; n = 1–3 per group from three independent experiments). (G) Left: Representative flow cytometry plots indicating VAT-T R frequency with or without CCL11/24 blockade. Graphs summarize T R frequencies in indicated tissues after 2 wk ( n = 3–4 per group). Mice were age matched within independent experiments and collectively; pooled data are from experiments using male mice aged 8–16 wk unless otherwise indicated. Statistical significance was determined using one-way ANOVA with Tukey’s post-test (A and C); two-tailed, paired Student’s t test for expression in donor cells within the same chimeric mouse (B); and two-tailed Student’s t test (F and G). All data are presented as mean values ± SD.

    Article Snippet: Mice aged 8–10 wk were given 0.75 µg/g body weight αCCL11 and αCCL24 (MAB420 and MAB528; R&D Systems) or an equivalent amount of rat IgG (Sigma) diluted in PBS by i.p. injection on days 0, 5, and 10 and sacrificed for analysis on day 13.

    Techniques: Expressing, In Vitro, Flow Cytometry, Two Tailed Test

    Increased accumulation of CCR3 + T R s in the absence of ICOS signaling is specific to VAT (goes with ). (A) CCR2 expression by splenic (top) and VAT-T R s (bottom) as measured by flow cytometry ( n = 3–5 per group from two independent experiments). (B) Expression of CCR3 in splenic T R s in mice ≤8 wk and >8 wk of age ( n = 3–5 per group from seven independent experiments). (C) Expression of CCR3 by gated CD45.1 + and CD45.2 + donor VAT-T R s in chimeric mice. Graphs summarize CCR3 expression by donor T R s in VAT and spleen. Line connects point representing CD45.1 + and CD45.2 + cells within the same chimeric mouse ( n = 2–4 per group from two independent experiments). (D) CCR3 expression by tissue-localized skin T R s as measured by flow cytometry ( n = 2–4 per group from two independent experiments). (E) CCR3 expression by CD45.1 + and CD45.2 + donor T R s within the same chimeric mouse in indicated tissues. Line connects CD45.1 + and CD45.2 + cells within the same chimeric mouse ( n = 2–4 per group from two independent experiments). (F) Frequency of tissue-restricted VAT eosinophils with and without in vivo CCL11/24 blockade as measured by flow cytometry ( n = 3 or 4 mice per group). Mice were age matched within individual experiments, and pooled data are from experiments using 8–16-wk-old male mice. Statistical significance was determined using one-way ANOVA with Tukey’s post-test (A, B, and D); two-tailed, paired Student’s t test for expression in donor cells within the same chimeric mouse (C and E); and two-tailed Student’s t test (F). All data are presented as mean values ± SD. LPL, lamina propria lymphocyte.

    Journal: The Journal of Experimental Medicine

    Article Title: ICOS signaling limits regulatory T cell accumulation and function in visceral adipose tissue

    doi: 10.1084/jem.20201142

    Figure Lengend Snippet: Increased accumulation of CCR3 + T R s in the absence of ICOS signaling is specific to VAT (goes with ). (A) CCR2 expression by splenic (top) and VAT-T R s (bottom) as measured by flow cytometry ( n = 3–5 per group from two independent experiments). (B) Expression of CCR3 in splenic T R s in mice ≤8 wk and >8 wk of age ( n = 3–5 per group from seven independent experiments). (C) Expression of CCR3 by gated CD45.1 + and CD45.2 + donor VAT-T R s in chimeric mice. Graphs summarize CCR3 expression by donor T R s in VAT and spleen. Line connects point representing CD45.1 + and CD45.2 + cells within the same chimeric mouse ( n = 2–4 per group from two independent experiments). (D) CCR3 expression by tissue-localized skin T R s as measured by flow cytometry ( n = 2–4 per group from two independent experiments). (E) CCR3 expression by CD45.1 + and CD45.2 + donor T R s within the same chimeric mouse in indicated tissues. Line connects CD45.1 + and CD45.2 + cells within the same chimeric mouse ( n = 2–4 per group from two independent experiments). (F) Frequency of tissue-restricted VAT eosinophils with and without in vivo CCL11/24 blockade as measured by flow cytometry ( n = 3 or 4 mice per group). Mice were age matched within individual experiments, and pooled data are from experiments using 8–16-wk-old male mice. Statistical significance was determined using one-way ANOVA with Tukey’s post-test (A, B, and D); two-tailed, paired Student’s t test for expression in donor cells within the same chimeric mouse (C and E); and two-tailed Student’s t test (F). All data are presented as mean values ± SD. LPL, lamina propria lymphocyte.

    Article Snippet: Mice aged 8–10 wk were given 0.75 µg/g body weight αCCL11 and αCCL24 (MAB420 and MAB528; R&D Systems) or an equivalent amount of rat IgG (Sigma) diluted in PBS by i.p. injection on days 0, 5, and 10 and sacrificed for analysis on day 13.

    Techniques: Expressing, Flow Cytometry, In Vivo, Two Tailed Test